Geoffrey Chang , Mechanosensitive Ion Channel : A GatedMycobacterium tuberculosis Structure of the MscL Homolog
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منابع مشابه
Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.
Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function ...
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Total chemical protein synthesis was used to generate multimilligram quantities of the mechanosensitive channel of large conductance from Escherichia coli (Ec-MscL) and Mycobacterium tuberculosis (Tb-MscL). Cysteine residues introduced to allow chemical ligation were masked with cysteine-reactive molecules, resulting in side chain functional groups similar to those of the wild-type protein. Syn...
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Mechanosensitive (MS) channels that provide protection against hypoosmotic shock are found in the membranes of organisms from the three domains of life: bacteria, archaea, and eucarya. Two families of ubiquitous MS channels are recognized, and these have been designated the MscL and MscS families. A high-resolution X-ray crystallographic structure is available for a member of the MscL family, a...
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This study aims to explore gating mechanisms of mechanosensitive channels in terms of membrane tension, membrane adaptation, protein conformation, and energetics. The large conductance mechanosensitive channel from Mycobacterium tuberculosis (Tb-MscL) is used as a model system; Tb-MscL acts as a safety valve by releasing small osmolytes through the channel opening under extreme hypoosmotic cond...
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The mechanosensitive channel of large conductance (MscL) serves as a model system for mechanosensitive ion channels gated by the bilayer mechanism. Here we report our measurements of the distance changes of multiple residues of MscL using smFRET. These measurements were then used to develop a molecular dynamics model for the open structure of the protein. We find that its open pore size is 2.8 ...
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